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Gibco™ Medium 199, Earle's Salts, powder DFS Item

Widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

Supplier:  Gibco™ 31100035

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Catalog No. 31100035



Description

Description

  • Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts
  • It has broad species applicability, particularly for cultivation of non-transformed cells
  • Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues
  • Store at 2°C to 8°C

Modified as follows:

  • With: L-glutamine, phenol red
  • Without: HEPES, sodium bicarbonate

Using Medium 199

  • Medium 199 contains no proteins, lipids, or growth factors
  • It uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
  • Compared to other basal media, Medium 199 contains unique components including adenine, adenosine, hypoxanthine, thymine, and additional vitamins
Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Powder
36 Months From Date of Manufacture
Low Glucose, Glutamine, Phenol Red
Gibco™
Room Temperature
Rat epithelial
Animal Origin-Free
Medium 199
Sterile-filtered
No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
10 x 1 L
SDS
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