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Gibco™ Medium 199, Earle's Salts, powder DFS Item

Originally developed for nutritional studies of chick embryo fibroblasts

Manufacturer:  Gibco™ LS31100035

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Catalog No. 31100035



Description

Description

Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

  • It is modified as follows: with L-glutamine and phenol red; without HEPES or sodium bicarbonate
  • Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Contains no proteins, lipids, or growth factors
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at time of preparation

Cell Culture, Mammalian Cell Culture

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
No HEPES
10 x 1L
No Sodium Pyruvate
L-Glutamine
Low Glucose
Gibco®
Room Temperature
Powder
Phenol Red
SDS
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