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Gibco™ Medium 199, Earle's Salts, powder 
Widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.
Supplier: Gibco™ 31100035
Description
- Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts
- It has broad species applicability, particularly for cultivation of non-transformed cells
- Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues
- Store at 2°C to 8°C
Modified as follows:
- With: L-glutamine, phenol red
- Without: HEPES, sodium bicarbonate
Using Medium 199
- Medium 199 contains no proteins, lipids, or growth factors
- It uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH
- Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
- Compared to other basal media, Medium 199 contains unique components including adenine, adenosine, hypoxanthine, thymine, and additional vitamins
Specifications
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
|
| Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues | |
| Powder | |
| 36 Months From Date of Manufacture | |
| Low Glucose, Glutamine, Phenol Red | |
| Gibco™ | |
| Room Temperature |
| Rat epithelial | |
| Animal Origin-Free | |
| Medium 199 | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate | |
| 10 x 1 L |