Gibco™ Medium 199, Earle's Salts, powder 

Description

Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

• It is modified as follows: with L-glutamine and phenol red; without HEPES or sodium bicarbonate
• Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
• Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
• Contains no proteins, lipids, or growth factors
• Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
• Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at time of preparation

Cell Culture, Mammalian Cell Culture