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Gibco™ Medium 199, Earle's Salts, powder 
Originally developed for nutritional studies of chick embryo fibroblasts
Manufacturer: Gibco™ LS31100035
Description
Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.
- It is modified as follows: with L-glutamine and phenol red; without HEPES or sodium bicarbonate
- Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
- Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
- Contains no proteins, lipids, or growth factors
- Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
- Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at time of preparation
Cell Culture, Mammalian Cell Culture
Specifications
| No HEPES | |
| Gibco™ | |
| L-Glutamine | |
| 10 x 1L | |
| No Sodium Pyruvate |
| Room Temperature | |
| Powder | |
| Phenol Red | |
| Low Glucose |