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Gibco™ Medium 199, Powder DFS Item

Originally developed for nutritional studies of chick embryo fibroblasts

$64.58 - $219.45

Specifications

Format Powder
Formulation Adenine sulfate, Adenosine 5-phosphate, Adenosine 5-triphosphate, Alpha-tocopherol Phosphate, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Cholesterol, Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Deoxyribose, Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Guanine hydrochloride, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Menadione (Vitamin K3), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Ribose, Sodium Acetate, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Thymine, Tween 80, Uracil, Vitamin A (acetate), Vitamin D2 (Calciferol), Xanthine-Na, i-Inositol
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

31100019

DFS Item
 
life technologies
31100019
50L Each for $219.45

31100035

DFS Item
 
life technologies
31100035
10 x 1L Each for $64.58
Description & Specifications

Specifications

Format Powder
Formulation Adenine sulfate, Adenosine 5-phosphate, Adenosine 5-triphosphate, Alpha-tocopherol Phosphate, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Cholesterol, Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Deoxyribose, Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Guanine hydrochloride, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Menadione (Vitamin K3), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Ribose, Sodium Acetate, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Thymine, Tween 80, Uracil, Vitamin A (acetate), Vitamin D2 (Calciferol), Xanthine-Na, i-Inositol
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture

Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

  • It is modified as follows: with L-glutamine and phenol red; without HEPES or sodium bicarbonate
  • Contains adenine, adenosine, hypoxanthine, thymine, and additional vitamins
  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Contains no proteins, lipids, or growth factors
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at time of preparation

Cell Culture, Mammalian Cell Culture

Shipping Condition: Room Temperature

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