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Gibco™ DPBS (10X), no calcium, no magnesium
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Catalog No. 14200075
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500 mL
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Catalog No. 14200075 Supplier Gibco™ Supplier No. 14200075
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Description

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ DPBS require pH adjustment and filtration at the time of preparation (see protocol for details).

We offer a variety of Gibco™ DPBS formulations for a range of cell culture applications.

This DPBS is modified as follows:

Without: Calcium, Magnesium, Phenol Red

The complete formulation is available.

Order Info

Shipping Condition: Room Temperature

Specifications

Chemical Name or Material DPBS
Color Clear
Concentration 10 X
For Use With (Application) Mammalian Cell Culture
pH 6.7 - 7.0
Physical Form Liquid
Product Line Gibco
Quantity 500 mL
Solution Type Dulbecco's Phosphate Buffered Saline
Sterility Sterile-filtered
Includes No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Osmolality 2630 - 3000 mOsm/kg
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Frequently Asked Questions (FAQs)

Do you offer an alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538)?

We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.

We recommend the following primary antibodies:

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)

alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)

beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)

We recommend the following secondary antibodies:

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)

https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121

We recommend using the following reagents:

https://www.thermofisher.com/order/catalog/product/88-8824-00

NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)

Blocker BSA (Cat. No. 37520)

DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)
Can I get a specific value for the conductivity for your DPBS products?

Sorry, we do not test the conductivity of our DPBS products and hence cannot provide a value or range.

Are your DPBS products DNase/RNase-free? Can I use them for RNA work?

Our DPBS products are for cell culture use and have not been tested for DNase/RNase activity. We do not recommend using these products for RNA work.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.