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Gibco™ DMEM, High Glucose, No Glutamine 
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine is used in cell culture processing applications.
$49.60 - $497.00
Specifications
| Format | Liquid |
|---|---|
| Formulation | Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol |
| Packaging | Sustainable packaging |
| Storage Requirements | Storage: 2-8° C. Protect from light |
| For Use With (Application) | Mammalian Cell Culture |
Disclaimers:
When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.
Requires: Supplementation
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
| 11960077 |
Gibco™
LS11960077 |
6 x 1000mL |
Each for $497.00
|
|
11960069 |
Gibco™
LS11960069 |
10 x 500mL |
Each for $474.00
|
|
11960051 |
Gibco™
LS11960051 |
1000mL |
Each for $90.50
|
|
11960044 |
Gibco™
LS11960044 |
500mL |
Each for $49.60
|
|
Description
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Order Info
Shipping Condition: Room Temperature
Compliance
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Class II Device U.S.
Specifications
| Liquid | |
| Sustainable packaging | |
| Mammalian Cell Culture | |
| High Glucose; Phenol Red |
| Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol | |
| Storage: 2-8° C. Protect from light | |
| DMEM media | |
| L-glutamine; Sodium Pyruvate; HEPES |