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Gibco™ DMEM, high glucose DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.

Supplier:  Gibco™ 11965126

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Catalog No. 11965126



  • DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
  • Store at 2°C to 8°C, protected from light

Modified as follows:

  • With: High glucose, L-glutamine, phenol red
  • Without: Sodium pyruvate, HEPES

Using DMEM

  • DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • It uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH


Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)
12 Months From Date of Manufacture
High Glucose, Glutamine, Phenol Red
Mammalian Cell Culture
Sustainable packaging
6 x 1000 mL
HeLa, 293, Cos-7, and PC-12
Animal Origin-Free
Standard Serum Supplementation
No HEPES, No Sodium Pyruvate
4500 mg/L




When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.