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Gibco™ DMEM, high glucose 
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.
$62.00 - $610.00
Specifications
| Concentration | 1X |
|---|---|
| Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
| Sterility | Sterile-filtered |
| Glucose Concentration | 4500 mg/L |
| Serum Supplementation | Standard Serum Supplementation |
Requires: Supplementation
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|
11965118
|
Gibco™
11965118 |
10 x 500 mL |
Case of 10 Each for $471.00
|
|
11965092
|
Gibco™
11965092 |
500 mL |
Each for $62.00
|
|
11965126
|
Gibco™
11965126 |
6 x 1000 mL |
Each for $334.00
|
|
11965084
|
Gibco™
11965084 |
1000 mL |
Each for $92.25
|
|
11965167
|
Gibco™
11965167 |
5 L |
Each for $413.00
|
|
11965175
|
Gibco™
11965175 |
10 L |
Each for $610.00
|
|
Description
- DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
- Store at 2°C to 8°C, protected from light
Modified as follows:
- With: High glucose, L-glutamine, phenol red
- Without: Sodium pyruvate, HEPES
Using DMEM
- DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- It uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH
Order Info
Shipping Condition: Room Temperature
Specifications
| 1X | |
| Sterile-filtered | |
| Standard Serum Supplementation | |
| L-Glutamine | |
| 12 Months From Date of Manufacture | |
| High Glucose, L-Glutamine, Phenol Red | |
| High Glucose | |
| HeLa, 293, Cos-7, and PC-12 | |
| DMEM (Dulbecco's Modified Eagle Medium) |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| 4500 mg/L | |
| Liquid | |
| Phenol Red | |
| Primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells | |
| HEPES, Sodium Pyruvate | |
| No Sodium Pyruvate | |
| Animal Origin-Free | |
| No HEPES |
When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.