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Gibco™ DMEM, high glucose DFS Item

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

$66.00 - $668.00

Specifications

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
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Products 6
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
11965092 DFS Item
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Gibco™
11965092
500 mL
Each for $66.00
 
11965118 DFS Item
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Gibco™
11965118
10 x 500 mL
Case of 10 Each for $502.00
 
11965084 DFS Item
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Gibco™
11965084
1000 mL
Each for $99.50
 
11965126 DFS Item
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Gibco™
11965126
6 x 1000 mL
Each for $363.00
 
11965167 DFS Item
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Gibco™
11965167
5 L
Each for $451.00
 
11965175 DFS Item
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Gibco™
11965175
10 L
Each for $668.00
 
Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red
Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Glutamine, Phenol Red
4500 mg/L
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)
Sterile-filtered
No HEPES, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
SDS
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.