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Gibco™ DMEM, high glucose DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.

$62.00 - $610.00

Specifications

Concentration 1X
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Sterility Sterile-filtered
Glucose Concentration 4500 mg/L
Serum Supplementation Standard Serum Supplementation
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Requires: Supplementation


Products 6
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
11965118 DFS Item
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Gibco™
11965118
10 x 500 mL
Case of 10 Each for $471.00
 
11965092 DFS Item
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Gibco™
11965092
500 mL
Each for $62.00
 
11965126 DFS Item
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Gibco™
11965126
6 x 1000 mL
Each for $334.00
 
11965084 DFS Item
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Gibco™
11965084
1000 mL
Each for $92.25
 
11965167 DFS Item
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Gibco™
11965167
5 L
Each for $413.00
 
11965175 DFS Item
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Gibco™
11965175
10 L
Each for $610.00
 
Description

Description

  • DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
  • Store at 2°C to 8°C, protected from light

Modified as follows:

  • With: High glucose, L-glutamine, phenol red
  • Without: Sodium pyruvate, HEPES

Using DMEM

  • DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • It uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

1X
Sterile-filtered
Standard Serum Supplementation
L-Glutamine
12 Months From Date of Manufacture
High Glucose, L-Glutamine, Phenol Red
High Glucose
HeLa, 293, Cos-7, and PC-12
DMEM (Dulbecco's Modified Eagle Medium)
DMEM (Dulbecco's Modified Eagle Medium)
4500 mg/L
Liquid
Phenol Red
Primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
HEPES, Sodium Pyruvate
No Sodium Pyruvate
Animal Origin-Free
No HEPES
SDS
Product Certifications

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.