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Thermo Scientific™ GFP-4E-BP1
Description
4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
For use in combination with the LanthaScreen™ Tb-anti-4E-BP1 [pThr46] Antibody (Cat. No. PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
Specifications
Specifications
| Assay Entry | Biochemical terbium phospho-specific activity |
| Content And Storage | GFP-4E-BP1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 2 mM DTT Store at -80°C. |
| Detection Method | Fluorescence |
| Form | Liquid |
| For Use With (Application) | Pharma and biopharma, protein assays and analysis, target and lead identification and validation |
| Quantity | 20 nmol |
| Substrate | 4E-BP1 |
| Substrate Properties | Protein-Based Substrate |
| Substrate Type | Kinase Substrate |
| Target Enzyme | Serine/Threonine Kinases |
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For Research Use Only. Not for use in diagnostic procedures.