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  6. Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Catalog No. p-3667204
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Quantity:
1 mL
5 x 1 mL
Particle Size:
45 to 165 μm
90 μm
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Catalog No. Quantity Particle Size
45001429 5 x 1 mL 90 μm
45000506 1 mL 45 to 165 μm
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Catalog No. 45001429 Supplier Cytiva Supplier No. 28401745
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Description

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 x 25mm are prepacked with Glutathione Sepharose™ 4B

  • Prepacked with Glutathione Sepharose™ 4B media, featuring a glutathione ligand coupled to 4% agarose
  • May be used with syringe, peristaltic pump or ¨A¨KTA design chromatography system
  • Purifies GST-tagged proteins from bacterial lysates and other glutathione S-transferases or glutathione-dependent proteins
  • Average particle size: 90μm
  • 1 and 5mL columns
Media
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: >25mg horse liver GST/mL
  • pH stability working range: 4 to 13

1mL columns

  • Bed dimensions: 7 × 25mm
  • Flow velocity: <4mL/min.

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.27 in.
Diameter (Metric) Inner 7 mm
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Flow Rate <4 mL/min.
Material Polypropylene
pH Range 4 to 13
Support Type 4% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 90 μm
Quantity 5 x 1 mL
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Type Pre-packed Column
Ligand Type Glutathione
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