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  6. Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

Catalog No. p-4892062
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5 x 5 mL
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45001431 5 x 5 mL
45001430 1 x 5 mL
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Catalog No. 45001431 Supplier Cytiva Supplier No. 28401748
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Description

For fast and simple purification of GST-tagged proteins

  • Prepacked with Glutathione Sepharose™ 4B media, featuring a glutathione ligand coupled to 4% agarose
  • May be used with syringe, peristaltic pump or ¨A¨KTA design chromatography system
  • Purifies GST-tagged proteins from bacterial lysates and other glutathione S-transferases or glutathione-dependent proteins
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.
  • Average particle size: 90μm
Media
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: >25mg horse liver GST/mL
  • pH stability working range: 4 to 13

Specifications

Bed Volume 5 mL
Diameter (English) Inner 0.62 in.
Diameter (Metric) Inner 16 mm
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Flow Rate <20 mL/min.
Material Polypropylene
pH Range 4 to 13
Support Type 4% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 45 to 165 μm
Quantity 5 x 5 mL
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
Type Pre-packed Column
Ligand Type Glutathione
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