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  5. Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Catalog No. T1029
Encompass
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T1029 200 Assays
T1027 1000 Assays
T1028 10,000 Assays
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Catalog No. T1029 Supplier Invitrogen™ Supplier No. T1029

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

Enzyme substrate and reaction buffer

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

  • Homogeneous assay format allows detection of β-galactosidase in presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible
  • The kinetics of glow-assay provides a window during which measurements may be performed, facilitating HTS applications where assay automation is used
  • Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
  • Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range
  • Assay sensitivity is 100 to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
  • Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
  • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
  • Assay can be completed in about one hour, providing fast assay turnaround

Ideal for Screening
This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

Specifications

  • Assay Type: β-Gal (lacZ) Assay
  • Compatible Cells: Mammalian Cells
  • Detection Method: Chemiluminescent
  • For Use With Equipment: Luminometer (Microplate)
  • Form: Liquid
  • Format: 96-well plate
  • Readout: End Point
  • Sample Type (Specific): Cell Cultures
  • Substrate: Galacton-Star™
  • Substrate Properties: Chemical Substrate
  • Substrate Type: Beta-Gal Substrate
  • Target Enzyme: Beta-Galactosidase
  • Technique: Enhanced Chemiluminescence

Specifications

Product Type β-Galactosidase Reporter Gene Assay System
Detection Method Chemiluminescence
For Use With (Equipment) Luminometer (Microplate)
Assay β-Gal (lacZ) Assay
Content And Storage This kit contains the enzyme substrate and the reaction buffer.
Store the product at 4°C.
Compatible Cells Mammalian Cells
High-throughput Compatibility High-throughput Compatible
Label or Dye Unconjugated
Readout End Point
Sample Type Cell Cultures
Substrate Galacton-Star™
Substrate Type Beta-Gal Substrate
Substrate Properties Chemical Substrate
Target Enzyme Beta-Galactosidase
Product Line Gal-Screen, NovaBright
For Use With (Application) Enzyme Assay
Quantity 200 Assays
Form Liquid
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For Research Use Only. Not for use in diagnostic procedures.