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Fluorescein-MAP2K1

Catalog No. PV4812
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20 nmol
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Catalog No. Quantity
PV4812 20 nmol
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Catalog No. PV4812 Supplier Thermo Fisher Scientific Supplier No. PV4812
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Description

Fluorescein-MAP2K1

Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members.

For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

Assay Entry Biochemical terbium phospho-specific activity
Content And Storage Fluorescein-MAP2K1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1mM DTT. Store at -80°C.
Detection Method Fluorescence
Quantity 20 nmol
Substrate MAP2K1
Substrate Properties Protein-Based Substrate
Substrate Type Kinase Substrate
Target Enzyme Serine/Threonine Kinases
Gene ID (Entrez) 10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885
Label or Dye FITC (Fluorescein)
Molecular Weight (g/mol) 50000 Da
Shipping Condition Dry Ice
Target Entry MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3
Validated Kinases BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11
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For Research Use Only. Not for use in diagnostic procedures.