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eBioscience Fixable Viability Dye eFluor™ 520

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

$114.55 - $419.05

Specifications

For Use With (Application) Flow Cytometric Analysis
Format DMSO, pre-diluted to test size
Storage Requirements ≤−70°C. Protect from light and moisture.
View More Specs

 Disclaimers

For Research Use Only

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501124899

 
ebioscience
65086718
500 tests Each for $419.05

501129208

 
ebioscience
65-0867-14
100 tests Each for $114.55
Description & Specifications

Specifications

For Use With (Application) Flow Cytometric Analysis
Format DMSO, pre-diluted to test size
Storage Requirements ≤−70°C. Protect from light and moisture.

  • Fixable Viability Dyes may be used to label cells from all species
  • Fixable Viability Dye eFluor™ 520 can be excited by the blue (488nm) laser line and has a peak emission of 522nm that can be detected using a 530/30 band pass filter (equivalent to FITC)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
  • Fixable Viability Dye eFluor™ 520 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
  • Store at −70°C with dessicant
  • It may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
  • Staining may be done before or after surface staining
  • Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing