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  6. Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520

Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520

Catalog No. 501129208
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501124899 500 tests
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Catalog No. 501129208 Supplier Invitrogen™ Supplier No. 65086714
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Description

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

  • Fixable Viability Dyes may be used to label cells from all species
  • Fixable Viability Dye eFluor™ 520 can be excited by the blue (488nm) laser line and has a peak emission of 522nm that can be detected using a 530/30 band pass filter (equivalent to FITC)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • Fixable Viability Dye eFluor™ 520 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
  • Store at −70°C with dessicant
  • It may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
  • Staining may be done before or after surface staining
  • Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing

Specifications

Color Green
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Dye
Emission 488/522
Product Line eBioscience
Quantity 100 tests

For Research Use Only. Not for use in diagnostic procedures.