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Cytiva First-Strand cDNA Synthesis Kit
For synthesis of single-stranded cDNA from mRNA templates prior to amplification by PCR
Supplier: Cytiva 27926101
Comprend: Bulk first-strand cDNA reaction mixes (Cloned murine reverse transcriptase, RNase inhibitor, RNase/DNase-free BSA, dATP, dCTP, dGTP, and dTTP in aqueous buffer), mRNA standard, DTT solution, RNase-free water, pd(N)6 primer, and Not I-(dT)19 bifunctional primer
Description
- Preassembled bulk reaction mixes save time and minimize potential for pipetting errors
- Primers are not included in the preassembled reaction mixes, allowing use of a primer of choice
- Two primers are included with the kit
- pd(N)6 primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail
- Not I-(dT)18 bifunctional primer can be used to prime selectively on mRNA having a poly(A) tail and can also be used as a PCR primer following first-strand synthesis
- The Not I restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products
Spécifications
| -15°C to -30°C | |
| RT-PCR | |
| 55 | |
| 55 Reactions |
| Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. | |
| Bulk first strand cDNA reaction mixes, mRNA standard, DTT solution, RNase-free Water, pd(N)6 primer and NotI-(dT)18 bifunctional primer | |
| First-Strand cDNA Synthesis Kit |