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Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for Ã-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Supplier: Thermo Scientific™ R0851
Description
X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).
Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).Recommended for:
- Detection of GUS expression in plant cells and tissues (1)
- Detection of infections caused by E.coli
- Detection of bacterial contamination in food and water samples
Note:
Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.
Specifications
| Colorimetric | |
| 0.1 g | |
| Beta-Glucuronidase (GUS) Substrate |
| X-Gluc | |
| Chemical Substrate |
For Research Use Only. Not for use in diagnostic procedures.