missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. Miscellaneous Molecular Biology Reagents
  5. Thermo Scientific™ X-Gluc

Thermo Scientific™ X-Gluc

Catalog No. FERR0851
Change view
Click to view available options
Quantity:
0.1 g
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FERR0851 0.1 g
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. FERR0851 Supplier Thermo Scientific™ Supplier No. R0851
Only null left

Description

Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Recommended for:

  • Detection of GUS expression in plant cells and tissues (1)
  • Detection of infections caused by E.coli
  • Detection of bacterial contamination in food and water samples

Note:

Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

Specifications

Detection Method Colorimetric
Product Type X-Gluc
Quantity 0.1 g
Substrate Properties Chemical Substrate
Substrate Type Beta-Glucuronidase (GUS) Substrate

For Research Use Only. Not for use in diagnostic procedures.