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Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.

$79.28 - $319.20

Specifications

Concentration 20U/µL
Inhibitors metal chelators, ammonium, chloride, iodide, phosphate ions
Source E. coli cells carrying a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase
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Products
Catalog Number Mfr. No. Units Price Quantity    

FEREP0161

 
thermo scientific™
EP0161
500U Each for $79.28

FEREP0162

 
thermo scientific™
EP0162
2,500U Each for $319.20
Description & Specifications

Specifications

Concentration 20U/µL
Inhibitors metal chelators, ammonium, chloride, iodide, phosphate ions
Source E. coli cells carrying a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase

Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA. TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.

Highlights

  • Incorporates modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)