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Thermo Scientific™ RNase I (10 U/μL)

Catalog No. feren0601
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Catalog No. FEREN0601 Supplier Thermo Scientific™ Supplier No. EN0601
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Description

Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.

Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates.

The enzyme does not require any metal ions for activity.

Highlights

  • Stable and active under a wide variety of reaction conditions
  • Can be heat inactivated in 30 minutes at 100°C

Specifications

Concentration 10 U/μL
Enzyme RNase
Quantity 1,000 units
Product Type RNase I

Frequently Asked Questions (FAQs)

What are your recommendations for removal of RNA from DNA solutions using Thermo Scientific RNase I?

1. Add 15-20 u of RNase I per 1 µg of RNA. RNase I is ≥ 90% active within pH range 7.0-8.8 at salt concentration 100-200 mM. Incubation with RNase I can be performed simultaneously with the digestion of DNA by restriction endonucleases.

2. Incubate at 37 degrees C for 30 minutes.

3. Purify DNA by spin column or phenol/chloroform extraction.

For Research Use Only. Not for use in diagnostic procedures.