RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.
- High yields of full-length first strand cDNA up to 13 kb
- Increased reaction temperatures in the range of 42 to 55°C
- Supplied with the recombinant RiboLock RNase Inhibitor
- Complete kit - oligo(dT)18 and random hexamer primers included with the kit
|First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.|
|RevertAid™ H Minus First Strand cDNA Synthesis Kit|