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  6. Thermo Scientific™ Rapid DNA Ligation Kit

Thermo Scientific™ Rapid DNA Ligation Kit

Catalog No. p-4530525
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50 Reactions
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FERK1422 50 Reactions
FERK1423 150 Reactions
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Catalog No. FERK1422 Supplier Thermo Scientific™ Supplier No. K1422
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Description

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.

The kit contains T4 DNA ligase and a specially formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.
  • Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
  • Convenient—reaction mixture can be used directly for bacterial transformation

Recommended for:

Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.

Note:

Lyophilized bacterial strains GM2163 (†M0099) and JM107 (†M0109) can be provided free of charge upon request, with any purchase. Prior to electroporation it is necessary to inactivate T4 DNA ligase (1) by chloroform extraction.

Specifications

Buffer 5X Rapid Ligation Buffer
Enzyme DNA Ligase
Product Type Rapid DNA Ligation Kit
Quantity 50 Reactions

For Research Use Only. Not for use in diagnostic procedures.