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  7. Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Catalog No. FERK0241
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No. of Reactions:
1,000 Reactions
200 reactions
4,000 Reactions
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Catalog No. No. of Reactions
FERK0241 200 reactions
FERK0243 4,000 Reactions
FERK0242 1,000 Reactions
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Catalog No. FERK0241 Supplier Thermo Scientific™ Supplier No. K0241
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Description

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix

Specifications

Concentration 2X
Content And Storage Contains:
• 2 x 1.25 mL Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with fluorescein passive reference dye; sufficient for 200 x 25 μL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Product Line Maxima
Product Type Real Time PCR SYBR Master Mix
Quantity 200 reactions
Sufficient For 200 Reactions of 25 μL
For Use With (Application) Gene Expression
No. of Reactions 200 reactions
Sample Type DNA, dsDNA
Volume 2 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.