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Thermo Scientific™ Genomic DNA Purification Kit

Catalog No. ferk0512
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FERK0512 100 Preps
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Catalog No. FERK0512 Supplier Thermo Scientific™ Supplier No. K0512
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Description

Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.

Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

  • Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
  • Fast—procedure takes approximately 20 minutes
  • Universal—purifies genomic DNA from various sources
  • Flexible—easy to scale up and down
  • Safe—does not involve phenol extraction

Specifications

Content And Storage • 40 mL Lysis Solution; room temperature
• 8 mL Precipitation Solution (concentrated); room temperature
• 10 mL NaCl Solution; room temperature
Isolation Technology Organic Extraction
Sample Type Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
Elution Volume 20 to 100 μL
Final Product Type gDNA
For Use With (Application) PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility Not High-throughput Compatible (Manual)
Quantity 100 Preps
Shipping Condition Room Temperature
Test Time 25 min.
Starting Material Amount Bacteria: ≤20 mg
Cells: ≤106
Plant: ≤100 mg
Tissue: ≤30 mg
Serum: ≤200 μL
Whole Blood: ≤200 μL
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For Research Use Only. Not for use in diagnostic procedures.