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Thermo Scientific™ GeneJET Viral DNA/RNA Purification Kit
Description
GeneJET Viral DNA and RNA Purification Kit is designed for rapid and efficient purification of high quality viral nucleic acids from various human and mammalian liquid samples, and is compatible with common sample preservatives like EDTA, citrate, and heparin.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column. Viral nucleic acids from lysed samples bind to the column membrane while impurities are effectively removed during subsequent washing and centrifugation steps. Ready-to-use nucleic acids are eluted from the column.
The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA and RNA can be directly used in PCR, qPCR, or other nucleic acid based assays.
Highlights:
- Efficient purification of viral DNA and RNA from human and mammalian plasma, serum, blood, and cell-free samples
- Linear yields from a wide range of viral titers
- Tested with a variety of DNA and RNA viruses (HIV, HBV, HCV, HPV, CMV, EBV)
- Downstream compatibility with (RT)-PCR, (RT)-qPCR, and other nucleic acid based assays.
Specifications
Specifications
| Column Type | Spin Column |
| Content And Storage | • 2 x 1.4 mL Column Preparation Liquid; room temperature • 12 mL Lysis Solution; room temperature • 25 mL Wash Buffer 1 (concentrated); room temperature • 11 mL Wash Buffer 2 (concentrated); room temperature • 3 x 1.25 mL Eluent; room temperature • 50 Spin Columns with Wash Tubes; room temperature • 4 x 50 Wash Tubes; room temperature • 2 x 1.3 mL Proteinase K; –20°C • 1 vial Carrier RNA; –20°C |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Milk, Plasma, Saliva, Serum, Swabs (Nasal, Buccal, or Urogenital), Urine, Whole Blood, Virus |
| Elution Volume | 50 μL |
| Final Product Type | Viral DNA and RNA |
| For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Quantity | 50 Preps |
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Frequently Asked Questions (FAQs)
We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.
We recommend performing centrifugation steps at room temperature when the temperature is not specified.
No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
For Research Use Only. Not for use in diagnostic procedures.