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Thermo Scientific™ GeneJET Plasmid Midiprep Kit
Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.
Supplier: Thermo Scientific™ K0482
Description
Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
- Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
- High concentration of DNA purified— >400 ng/μL
- Convenient—spin column format, centrifuge or vacuum protocols
- Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
- Transfection of robust cell lines
Specifications
| 400 ng/μL | |
| Spin Column | |
| Midi | |
| Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR | |
| 100-200 μg (Medium-Scale) Plasmid DNA | |
| Plasmid MidiPrep Kit | |
| Plasmid DNA |
| Spin Column | |
| Silica Column | |
| Bacteria | |
| Not High-throughput Compatible (Manual) | |
| GeneJET | |
| 100 Preps | |
| 60 min. |
For Research Use Only. Not for use in diagnostic procedures.