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  5. Thermo Scientific™ DTT (dithiothreitol)

Thermo Scientific™ DTT (dithiothreitol)

Catalog No. FERR0861
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5 g
25 g
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FERR0861 5 g
FERR0862 25 g
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Catalog No. FERR0861 Supplier Thermo Scientific™ Supplier No. R0861
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Description

Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.

DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state .

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (1). At a final 0.1M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.
  • Free of endo-, exodeoxyribonucleases, ribonucleases and phosphatases

Recommended for:

Enzyme stabilization; Enzymatic reactions; Protein electrophoresis.

Note:

DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1M DTT solution, dissolve 1.55g of DTT powder in 10mL of deionized water (e.g., Water, nuclease-free).

Specifications

Concentration 0.1 M
Product Type DTT (Dithiothreitol)
Quantity 5 g

Frequently Asked Questions (FAQs)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%
How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

For Research Use Only. Not for use in diagnostic procedures.