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Thermo Scientific™ 5-Fluoroorotic Acid

Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.

$105.00 - $417.90

Specifications

For Use With (Application) Detection of URA3 gene expression in yeast; Construction of yeast 2-hybrid libraries (1); Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mu
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

FERR0811

 
thermo scientific™
R0811
1g Each for $105.00

FERR0812

 
thermo scientific™
R0812
5g Each for $417.90
Description & Specifications

Specifications

For Use With (Application) Detection of URA3 gene expression in yeast; Construction of yeast 2-hybrid libraries (1); Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mu

5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).

Convertion of 5-FOA to fluorodeoxyuridine

Convertion of 5-FOA to fluorodeoxyuridine