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Invitrogen™ Mouse Fc gamma R2B (FCGR2B) ELISA Kit
ELISA
$908.00
Specifications
| Accession Number | P08101 |
|---|---|
| Assay Range | 0.096 to 70 ng/mL |
| Assay Sensitivity | 80 pg/mL |
| Calibration Range | 80 pg/mL to 70 ng/mL |
| Assay | Solid-phase sandwich ELISA |
Description
The Mouse Fc gamma R2B (Ms FCGR2B) ELISA quantitates Ms FCGR2B in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms FCGR2B. Principle of the method The Mouse FCGR2b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.Specifications
| P08101 | |
| 80 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| Mouse | |
| Colorimetric Microplate Reader | |
| AI528646,CD32,F630109E10Rik,Fc[g]RII,FcgRII,Fcgr2,Fcgr2a,Fcr-2,Fcr-3,Ly-17,Ly-m20,LyM-1,Lym-1,fcRII | |
| <10 | |
| HRP | |
| Immunology | |
| 2°C to 8°C | |
| CD32b (FCGR2B) | |
| 4 hrs 45 mins |
| 0.096 to 70 ng/mL | |
| 80 pg/mL to 70 ng/mL | |
| HRP | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 14130 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Plasma 50 μL, Serum 50 μL, Supernatant 100 μL | |
| AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1, fcRII | |
| 1 hrs 20 mins |