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Invitrogen™ Mouse Fc gamma R2B (FCGR2B) ELISA Kit

ELISA

Supplier:  Invitrogen™ EMFCGR2B

Encompass

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Catalog No. EMFCGR2B


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Description

Description

The Mouse Fc gamma R2B (Ms FCGR2B) ELISA quantitates Ms FCGR2B in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms FCGR2B. Principle of the method The Mouse FCGR2b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Specifications

Chemical Identifiers

P08101
80 pg/mL
HRP
Other Proteins
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
14130
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1, fcRII
1 hrs 20 mins
Solid-phase sandwich ELISA
0.096 to 70 ng/mL
80 pg/mL to 70 ng/mL
ELISA
Plasma, Serum, Supernatant
Mouse
Colorimetric Microplate Reader
AI528646,CD32,F630109E10Rik,Fc[g]RII,FcgRII,Fcgr2,Fcgr2a,Fcr-2,Fcr-3,Ly-17,Ly-m20,LyM-1,Lym-1,fcRII
<10
HRP
Immunology
2°C to 8°C
CD32b (FCGR2B)
4 hrs 45 mins

Specifications

P08101
80 pg/mL
Solid-phase sandwich ELISA
ELISA
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
14130
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Immunology
2°C to 8°C
CD32b (FCGR2B)
4 hrs 45 mins
0.096 to 70 ng/mL
80 pg/mL to 70 ng/mL
HRP
Other Proteins
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Mouse
Colorimetric Microplate Reader
AI528646,CD32,F630109E10Rik,Fc[g]RII,FcgRII,Fcgr2,Fcgr2a,Fcr-2,Fcr-3,Ly-17,Ly-m20,LyM-1,Lym-1,fcRII
<10
HRP
RUO
Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1, fcRII
1 hrs 20 mins
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