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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Supplier:  Thermo Scientific™ A39256

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Catalog No. PIA39256


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Description

Description

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

  • Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
  • Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
  • Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
  • Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
  • Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
  • Very short - spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.
Specifications

Specifications

10 x 1 mg
10 x 1 mg
Chemical Labeling
EZ-Link
Amine
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Short
Sulfo-NHS-Biotin
Store desiccated at -20°C.
Qualified for Ambient or Wet Ice
Cell-Impermeant
Sulfo-NHS Ester
Biotin
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For Research Use Only. Not for use in diagnostic procedures.