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Gibco™ ExpiSf™ Protein Production Kit

Catalog No. A3767806
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Quantity:
1 L (ExpiSf CD Medium), 4 x 1 mL (ExpiSf Enhancer)
10 L (ExpiSf CD Medium), 40 mL (ExpiSf Enhancer)
5 x 10 L (ExpiSf CD Medium), 5 x 40 mL (ExpiSf Enhancer)
6 x 1000 mL (ExpiSf CD Medium), 24 x 1 mL (ExpiSf Enhancer)
Catalog No. Quantity
A3767806 1 L (ExpiSf CD Medium), 4 x 1 mL (ExpiSf Enhancer)
A3767809 6 x 1000 mL (ExpiSf CD Medium), 24 x 1 mL (ExpiSf Enhancer)
A3767807 10 L (ExpiSf CD Medium), 40 mL (ExpiSf Enhancer)
A3767808 5 x 10 L (ExpiSf CD Medium), 5 x 40 mL (ExpiSf Enhancer)
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Catalog No. A3767806

Supplier: Gibco™ A3767806

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Description

Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.

The ExpiSf Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells. The kit includes the chemically defined, animal origin-free ExpiSf Enhancer and ExpiSf CD Medium, which together power the highest possible level of protein expression from baculovirus-infected ExpiSf9 Cells.

The ExpiSf Protein Production Kit:

  • Delivers greater than 80% more protein yield in high-density suspension Sf9 cell culture than ExpiSf CD Medium alone
  • Includes ExpiSf Enhancer, which is specifically designed to work in concert with ExpiSf CD Medium to significantly boost protein expression from baculovirus-infected insect cell cultures
  • Demonstrates direct scalability of baculovirus infection from culture volumes of 4 mL in deep-well plates to >3 L in shake flasks

The ExpiSf Protein Production Kit is specifically designed to work with ExpiSf9 Cells in the ExpiSf Expression System. The kit supplies sufficient ExpiSf Enhancer and ExpiSf CD Medium to infect 1 L, 10 L, or 50 L of culture. ExpiSf CD Medium is also available for purchase separately.

Specifications

Type Media and reagents, ExpiSf CD Meidum and ExpiSf Enhancer
Quantity 1 L (ExpiSf CD Medium), 4 x 1 mL (ExpiSf Enhancer)
Content And Storage 1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C; 4 x 1 mL ExpiSf Enhancer, store (at 2°C to 8°C; Shelf Life: 12 months from date of manufacture)
Classification Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Compatible Cells Insect Cells
Format Liquid
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Expression Mechanism Cell-Based Expression
Expression System Baculovirus
Product Line ExpiSf