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Gibco™ Expi293™ MembranePro™ Expression System

Catalog No. p-7075865
Encompass
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A25869 10 Reactions
A25870 100 Reactions
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Catalog No. A25869 Supplier Gibco™ Supplier No. A25869

Description

Expi293™ MembranePro™ Expression System

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.

Features of the Expi293™ MembranePro™ Expression System include:
  • Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system
  • Scalable—easy scale-up without the need for multiple flasks
  • Easy to use—no enzymatic or mechanical dissociation required

    In addition, benefits of MembranePro™ technology include:
  • Proteins are displayed on human (or other mammalian) cell membranes
  • Cellular quality control and mammalian post-translational processing helps produce functional protein
  • Proteins bud off as lipoparticles and are easily collected from the culture medium
  • Lipoparticles are enriched with expressed receptors

    With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
    1. Clone your gene of interest using a TOPO™ vector
    2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
    3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
    4. Store lipoparticles at -80 C or proceed to downstream assays

    Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.

    • Specifications

    Type Expression System
    Sufficient For 10 Reactions
    Delivery Type Virus-Like Particles (VLPs)
    Quantity 10 Reactions
    Content And Storage • MembranePro™ Reagent, 1 x 0.3 mg (1 mg/mL), store at -5 to -30°C
    • MembranePro™ Precipitation Mix, 1 x 75 mL, store at room temperature
    • 1 ExpiFectamine™ 293 transfection kit (containing reagent and enhancers) for 1 L of culture, store at 2–8°C
    • Opti-MEM™ I Reduced Serum Medium, 1 x 100 mL, store at 2–8°C
    • Expi293™ Expression Medium, 1 x 1 L, store in the dark at 2–8°C
    Cell Line Expi293F™
    Product Line Expi293, MembranePro
    For Use With (Application) Membrane Protein Expression
    Promoter EF-1α

    For Research Use Only. Not for use in diagnostic procedures.