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Gibco™ Expi293™ MembranePro™ Expression System
Expi293™ MembranePro™ Expression System
Supplier: Gibco™ A25870
Description
For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.Features of the Expi293™ MembranePro™ Expression System include:
In addition, benefits of MembranePro™ technology include:
With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO™ vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays
Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.
Specifications
Expression System | |
Virus-Like Particles (VLPs) | |
• MembranePro™ Reagent, 10 x 0.3 mg (1 mg/mL), store at -5 to -30°C • MembranePro™ Precipitation Mix, 10 x 75 mL, store at room temperature • 10 ExpiFectamine™ 293 transfection kits (containing reagent and enhancers) each for 1 L of culture, store at 2–8°C • Opti-MEM™ I Reduced Serum Medium, 10 x 100 mL, store at 2–8°C • Expi293™ Expression Medium, 10 x 1 L, store in the dark at 2–8°C |
|
Expi293™, MembranePro™ | |
EF-1α |
100 Reactions | |
100 Reactions | |
Expi293F™ | |
Membrane Protein Expression |
For Research Use Only. Not for use in diagnostic procedures.