Gibco™ Expi293™ Inducible Expression System Kit

• System kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression
• Inducible cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter
• This cell line is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins

Features:

• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

• 1 vial of frozen Expi293F Inducible cells
• 1 L of Expi293 Expression Medium
• 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
• 100 mL Opti-Plex Complexation Buffer
• 1 pcDNA5/TO Mammalian Expression Vector kit
• 500 mg tetracycline hydrochloride
• pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 Inducible Expression System are also available for individual purchase