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Gibco™ Expi293™ GnTI- Expression System Kit DFS Item

Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells

Manufacturer:  Gibco™ LSA39250

Catalog No. A39250



System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.


  • Homogeneous N-glycosylation of expressed proteins
  • Equivalent protein yields to parental Expi293F cells - up to 1 g/L
  • Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
  • Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
  • High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

  • 1 vial of frozen Expi293F GnTI- cells
  • 1 L of Expi293 Expression Medium
  • 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
  • 100 mL Opti-Plex Complexation Buffer
  • 1 PNGase F Glycan Cleavage kit
  • pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 GnTI- Expression System are also available for individual purchase



1 vial GnTI- cells; 1 L Expression medium; 1 transfection kit; 100 mL complexation buffer; 1 cleavage kit; positive control vector

For Research Use Only. Not for use in diagnostic procedures.