missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. In vitro Protein Expression Systems
  6. A39250

Gibco™ Expi293™ GnTI- Expression System Kit

Catalog No. A39250
Encompass
Change view
Click to view available options
Quantity:
1 kit
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A39250 1 kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A39250 Supplier Gibco™ Supplier No. A39250
Only null left

Description

Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells

System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Features:

  • Homogeneous N-glycosylation of expressed proteins
  • Equivalent protein yields to parental Expi293F cells - up to 1 g/L
  • Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
  • Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
  • High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

Components included:

  • 1 vial of frozen Expi293F GnTI- cells
  • 1 L of Expi293 Expression Medium
  • 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
  • 100 mL Opti-Plex Complexation Buffer
  • 1 PNGase F Glycan Cleavage kit
  • pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 GnTI- Expression System are also available for individual purchase

Specifications

Delivery Type Transfection
Quantity 1 kit
Content And Storage • Expi293F GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• PNGase F Glycan Cleavage Kit, store at -20°C
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Cell Line Expi293F™
Product Line Expi293
For Use With (Application) Protein Production

Frequently Asked Questions (FAQs)

Can I leave my Expi293F culture expressing for more than 7 days?

The growth and expression characteristics of Expi293F cells are such that, by 7 days post-transfection, the culture medium should be close to being spent and maximal protein expression should have already been achieved. Continued incubation will result in a large decrease in cell culture viability.
What method do you recommend for quantifying rabbit IgG expression from pRABBIT IgG IRES-EmGFP Positive Control Vector, Cat. No. A39243?

The formation of intact IgG molecules may be quantified using a sandwich ELISA designed to capture and detect rabbit IgG. Besides the rabbit IgG positive control vector, reagents, and consumables that are included in the kit, you will also need purified rabbit IgG to be used as a standard, F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Cat. No. A10547), Protein A Coated Plates, Clear, 96-Well (Cat. No. 15130), TMB Substrate Kit (Cat. No. 34021), SuperBlock (TBS) Blocking Buffer (Cat. No. 37535), and PBS or TBS buffer for washes. There is an example procedure in our Protein A Coated Plates manual (https://tools.thermofisher.com/content/sfs/manuals/MAN0011310_Thermo Scientific_ProteinA_G_AG_Coat_96Well_UG.pdf). Please note that our R&D scientists determine titer values from crude cell culture supernatants using a Pall Life Sciences FortéBio Octet instrument equipped with a Protein A biosensor.
Do you offer a GFP-expressing mammalian expression vector that I can use as a control to monitor my transfection and expression with the Expi systems?

We offer pRABBIT IgG IRES-EmGFP Positive Control Vector, Cat. No. A39243, which you can use to monitor your transfection and expression.

For Research Use Only. Not for use in diagnostic procedures.