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Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL)
Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.
Supplier: Thermo Scientific™ EN0591
Description
Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus. The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA . Endo IV possesses also a 3'≥5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing conditions. Substrates with 3'-recessed ends are preferred substrates for the 3'≥5' exonuclease activity .
The enzyme has no requirement for Mg2+ and is fully active in the presence of EDTA in moderate concentrations.
Applications- Studies of DNA damage and repair
- Single cell electrophoresis (comet assay)
- Antitumor drug research
- DNA structure research
- SNP analysis
Specifications
| 2 U/μL | |
| Endonuclease | |
| 100 units | |
| Endonuclease IV |
For Research Use Only. Not for use in diagnostic procedures.