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  6. Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Catalog No. pi21555
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50 mg
10 x 2 mg
1 g
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Catalog No. Quantity
PI21555 1 g
PIA39267 10 x 2 mg
PI21655 50 mg
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Catalog No. PI21555 Supplier Thermo Scientific™ Supplier No. LSG21555
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Description

Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.

Thermo Scientific™ Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

Highlights:

  • Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Crosslink oriented antibodies to Protein A or G agarose
  • Immobilize proteins onto amine-coated surfaces

Specifications

Chemical Reactivity Amine-Amine
Cleavable No
Description DSS
Molecular Weight (g/mol) 368.35
PEGylated No
Spacer Arm Length 11.4 Å
Content And Storage Upon receipt store desiccated at 4°C.
Cell Permeability Yes
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety NHS Ester
Spacer Medium (10 to 30 Å)
Form Powder
Quantity 1 g
Solubility DMF, DMSO
Format Standard, Single-use
Water Soluble No
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.