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  7. Thermo Scientific™ DreamTaq PCR Master Mixes (2X)

Thermo Scientific™ DreamTaq PCR Master Mixes (2X)

Catalog No. FERK1071
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Color:
Colorless
Green
Quantity:
4 x 1.25 mL
20 x 1.25 mL
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Catalog No. Color Quantity
FERK1071 Colorless 4 x 1.25 mL
FERK1072 Colorless 20 x 1.25 mL
FERK1081 Green 4 x 1.25 mL
FERK1082 Green 20 x 1.25 mL
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Catalog No. FERK1071 Supplier Thermo Scientific™ Supplier No. K1071
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Description

Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.

Thermo Scientific DreamTaq PCR Master Mix (2X), available in colorless and green formats, is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR setup.

The master mix retains all features of DreamTaq DNA Polymerase and is capable of robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

DreamTaq Green PCR Master Mix (2X) additionally includes 2X DreamTaq Green Buffer with a density reagent and two dyes for monitoring electrophoresis progress.

Features

  • Robust amplification with minimal optimization
  • High yields of PCR products
  • Higher sensitivity compared to conventional Taq DNA polymerase
  • Amplification of targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
  • Incorporates modified nucleotides, but does not incorporate dUTP
  • Direct loading of PCR product on gel with green format

Applications

  • Routine PCR amplification of DNA fragments up to 6 kb
  • High throughput PCR
  • Genotyping

Specifications

Concentration 5 U/μL
Content And Storage • DreamTaq PCR Master Mix (2X), 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL

Store at -20°C.
GC-Rich PCR Performance Low
Polymerase DreamTaq DNA Polymerase
Reaction Speed Standard
Quantity 4 x 1.25 mL
Shipping Condition Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
Overhang 3'-A
Reaction Format SuperMix or Master Mix
Color Colorless
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Frequently Asked Questions (FAQs)

What is the error rate of DreamTaq DNA Polymerases?

The error rate of DreamTaq DNA Polymerase is the same as that of Taq DNA Polymerase: 2.2x10^-5 errors per nt per cycle.
Can I do PCR directly from bacterial colonies with Thermo Scientific Taq Master Mixes?

Dream Taq PCR Master Mix and Taq PCR Master Mix can be used for direct PCR from bacterial colonies. Simply scrape a tiny amount of a single colony and transfer to a PCR-tube, prefilled with the master mix. Always use at least 3 min at 95 degrees C for initial denaturation.

For Research Use Only. Not for use in diagnostic procedures.