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Thermo Scientific™ DNase I, RNase-free (1 U/μL)

Catalog No. FEREN0521
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1,000 units
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FEREN0521 1,000 units
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Catalog No. FEREN0521 Supplier Thermo Scientific™ Supplier No. EN0521
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Description

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.

  • In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
  • In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides

Highlights

  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases

Applications

  • Preparation of DNA-free RNA
  • Removal of template DNA followingin vitrotranscription
  • Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
  • DNA labeling by nick-translation in conjunction with DNA Polymerase I
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications

Concentration 1 U/μL
Enzyme DNase
Compatible Buffer Reaction Buffer
Quantity 1,000 units
Product Type DNase I RNase Free

For Research Use Only. Not for use in diagnostic procedures.