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Thermo Scientific™ DNAPac™ RP Columns
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Catalog No. DX088924
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Length (Metric):
10 mm
50 mm
100 mm
150 mm
Diameter (Metric):
2.1 mm
3 mm
21.2 mm
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Catalog No. Length (Metric) Diameter (Metric)
DX088924 50 mm 2.1 mm
DX088919 100 mm 3 mm
DX088920 50 mm 3 mm
DX088921 10 mm 3 mm
DX088923 100 mm 2.1 mm
DX088925 10 mm 2.1 mm
03100098 10 mm 21.2 mm
03100097 150 mm 21.2 mm
95000 10 mm 21.2 mm
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Catalog No. DX088924 Supplier Thermo Scientific™ Supplier No. 088924
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Description

Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.

Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs.

High Performance Reversed-Phase LC Columns

The DNAPac RP column is based on spherical wide-pore 4μm polymer resin. The phase is highly stable to extremes of pH (0-14), and temperature (5-110°C), and operates with mobile phases compatible with direct mass spectrometry. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments. DNAPac RP reversed phase (RP) column is specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over.


Columns designed for reversed phase oligonucleotide LC and LC/MS separations
  • Designed for ion-pair reversed-phase (IP-RP) separations of both oligonucleotides and double-stranded nucleic acids
  • High resolution and high throughput
  • Excellent MS compatibility
  • Wide operating pH range (0-14) and high temperature stability (up to 110°C)

Specifications

Column Format Analytical Column
Diameter (Metric) 2.1 mm
Length (Metric) 50 mm
Max. Pressure 4000 psi (275 bar)
Particle Size 4 μm
pH 0 to 14
Pore Size Proprietary Wide Pore
Quantity 1 Ea.
Temperature <110°C
Stationary Phase DVB (Divinylbenzene) Polymer
Product Line DNAPac
Packing Material DVB (Divinylbenzene) Polymer
Column Type Reversed Phase
Phase Reversed Phase
Technique Bio-LC
Green Features FSC
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