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Thermo Scientific™ DNA Polymerase I

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

$94.00 - $375.00

Specifications

Concentration 10U/µL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (5)
Molecular Weight 103kDa monomer
Source E.coli cells with a cloned polA gene
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Description & Specifications

Specifications

Concentration 10U/µL
Inhibitors metal chelators, PPi, Pi (at high concentrations) (5)
Molecular Weight 103kDa monomer
Source E.coli cells with a cloned polA gene

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

  • Incorporates modified nucleotides (e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
  • Active in multiple buffers, including restriction enzyme, PCR, and RT buffers