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MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 3 μm Particle Size
Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.
Supplier: MilliporeSigma™ Supelco™ 567226U
Description
Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery™ BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery™ BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry.Greener Choice Claims
- Environmental benefits include:
- Renewable Energy
Specifications
2.1 mm | |
3 μm | |
100 m2/g | |
Discovery | |
Yes | |
Wide Pore HPLC |
5 cm | |
300 Å | |
C5 (pentyl) | |
C5 (pentyl) Phase/Silica Particle Platform | |
Reversed Phase |
Discovery is a registered trademark of Sigma-Aldrich Co. LLC