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Corning™ HEPES, Liquid

An alternative to use of bicarbonate for buffering

Manufacturer:  Corning™ 25060CI

Catalog No. MT25060CI



Description

Description

Technical Advantage:  Product manufactured under industry-leading standards for low endotoxin content and superior results.
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2.  Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer.  Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.  The recommended working concentration is 10-25 mM.
-  Complete Certificate of Analysis available for each production lot, along with full formulation information

Corning media certificates are available on our Resource Library.
Specifications

Specifications

Liquid
1M HEPES
Sterile-filtered
6 x 100 mL
7.2 to 7.6
15° to 30°C
SDS
Documents