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  6. Cytiva Con A Sepharose™ 4B Medium

Cytiva Con A Sepharose™ 4B Medium

Numéro de catalogue. p-3667108
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5 mL
100 mL
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Numéro de catalogue. Quantity
45000069 5 mL
45000068 100 mL
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Numéro de catalogue. 45000069 Fournisseur Cytiva Code fournisseur 17044003
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Description

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

  • Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars
  • Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
  • Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
  • Average particle size: 90μm
  • Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol

Spécifications

Binding Capacity 30mg/mL
Ligand Concentration 13mg Con A/mL Drained Medium
Matrix 4% Agarose
Particle Size 90 μm
pH 4 to 9
Quantity 5 mL
Content And Storage 2°C to 8°C
For Use With (Application) For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
Ligand Concanavalin A