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CCF4-AM

Catalog No. p-7075671
Encompass
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20mg
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Catalog No. Quantity
K1029 20mg
K1028 5mg
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Catalog No. K1029 Supplier Thermo Fisher Scientific Supplier No. K1029

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Description

CCF4-AM

CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer™ technology.

Specifications

Cell Permeability Cell-Permeant
Content And Storage Store in freezer (-5 to -30°C).
Detection Method Fluorescence
Form Solid
Product Line LiveBLAzer
Quantity 20mg
Substrate CCF4-AM
Substrate Properties Chemical Substrate
Substrate Type Beta-Lactamase Substrate
For Use With (Equipment) Flow Cytometer, Microplate Reader
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.