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CCF2-AM

Catalog No. K1023
Encompass
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5 mg
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K1023 5 mg
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Catalog No. K1023 Supplier Thermo Fisher Scientific Supplier No. K1023

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

CCF2-AM

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer™ technology.

Specifications

Cell Permeability Cell-Permeant
Content And Storage Store in freezer (-5 to -30°C).
Detection Method Fluorescence
Product Line GeneBLAzer
Quantity 5 mg
Substrate CCF2-AM
Substrate Properties Chemical Substrate
Substrate Type Beta-Lactamase Substrate
For Use With (Equipment) Flow Cytometer, Microplate Reader
Shipping Condition Room Temperature

For Research Use Only. Not for use in diagnostic procedures.