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CCF2-AM

Catalog No. K1023
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5 mg
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Catalog No. Quantity
K1023 5 mg
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Catalog No. K1023 Supplier Thermo Fisher Scientific Supplier No. K1023

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

CCF2-AM

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer™ technology.

Specifications

Cell Permeability Cell-Permeant
Content And Storage Store in freezer (-5 to -30°C).
Detection Method Fluorescence
Product Line GeneBLAzer
Quantity 5 mg
Substrate CCF2-AM
Substrate Properties Chemical Substrate
Substrate Type Beta-Lactamase Substrate
For Use With (Equipment) Flow Cytometer, Microplate Reader
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
- LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
- LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).

For Research Use Only. Not for use in diagnostic procedures.