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Thermo Scientific™ CaptureSelect™ LambdaXP Affinity Matrix

Catalog No. 2943752005
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2943752005 5 mL
194375201L 1 L
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Catalog No. 2943752005 Supplier Thermo Scientific™ Supplier No. 29 437 52005
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Description

Thermo Scientific™ CaptureSelect™ LambdaXP Affinity Matrix was specifically designed for the purification of recombinant human immunoglobulins and Fab fragments containing a lambda light chain.

CaptureSelect LambdaXP Affinity Matrix was specifically designed for the purification of recombinant human immunoglobulins and Fab fragments containing a lambda light chain. The ligand binds to the constant domain of the human lambda light chain and recognizes all isotypes including IgA, IgM, IgD, and IgE.

Features of this affinity matrix include:

  • Efficient elution of Fab fragments and immunoglobulins containing a lambda light chain, no cross binding to the Fc-region or kappa light chains
  • One-step purification from crude material with high purity and yield
  • Excellent scalability
  • Non-animal-derived

CaptureSelect LambdaXP Affinity Matrix can be utilized for the purification of human Ig's and Fab/Fab2 fragments directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in a single step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect LambdaXP
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >30 g IgG/L resin depending on flow rate, column height, and contact time
  • Elution conditions: 25 mM sodium acetate, pH 3.5
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity >30 g IgG/L resin
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Formulation 50% slurry suspension in 20% ethanol
Matrix Agarose-based, epoxide-activated
Content And Storage Store in refrigerator (2°C to 8°C), protect from daylight.
Max. Operating Pressure 2 bar
Shipping Condition Ships at 2°C to 8°C
Particle Size 65 ±10 μm
Form Agarose Bead
Product Line CaptureSelect
Quantity 5 mL
For Use With (Application) Lambda Fab purification
Target Lambda light chain
Type Affinity Matrix
Ligand Type LambdaXP
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