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Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix
Description
CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.
Features of this affinity matrix include:
- Purification of human IgE, binding different recombinant IgE forms
- Efficient removal of host cell proteins (HCP) and IgE aggregates
- Excellent scalability
- Non–animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
- Matrix: agarose-based, epoxide-activated
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect IgE affinity ligand
- Ligand coupling method: aldehyde coupling
- Binding capacity: >5 mg/mL resin
- Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Binding Capacity | >5 mg/mL resin |
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide-activated |
| Particle Size | 65 ±10 μm |
| Form | Resin Slurry |
| Product Line | CaptureSelect |
| Quantity | 1 L |
| Type | Affinity Matrix |
| Ligand Type | IgE affinity ligand |
| Stationary Phase | IgE |