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Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix
Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.
Supplier: Thermo Scientific™ 194354201L
Description
CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.
Features of this affinity matrix include:
- Purification of human IgE, binding different recombinant IgE forms
- Efficient removal of host cell proteins (HCP) and IgE aggregates
- Excellent scalability
- Non–animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
- Matrix: agarose-based, epoxide-activated
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect IgE affinity ligand
- Ligand coupling method: aldehyde coupling
- Binding capacity: >5 mg/mL resin
- Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20% (v/v) ethanol
Specifications
| >5 mg/mL resin | |
| Agarose-based, epoxide-activated | |
| Resin Slurry | |
| 1 L | |
| IgE affinity ligand |
| 50 to 200 cm/hr. (Up to 2 bar) | |
| 65 ±10 μm | |
| CaptureSelect™ | |
| Affinity Matrix | |
| IgE |