Thermo Scientific™ CaptureSelect™ FcXL Affinity Matrix

Features of this affinity matrix include:

• Mild elution, making it suitable for Fc fusion proteins
• Human specific, no binding to bovine antibodies, for example
• Excellent scalability
• Non-animal-derived

CaptureSelect FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main Characteristics:

• Matrix: agarose-based, aldehyde-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect FcXL affinity ligand
• Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
• Binding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl₂, 40% (v/v) propylene glycol, pH 5–6
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol